Journal: Cell Reports

Article Title: Microglial identity and inflammatory responses are controlled by the combined effects of neurons and astrocytes

doi: 10.1016/j.celrep.2021.108882

Figure Lengend Snippet:

Article Snippet: 3 days after microglial seeding, the media was replaced with pHrodoTM Red E. Coli BioParticlesTM conjugate suspension (1:10 dilution in PBS) prepared following manufacturer’s instructions (ThermoFisher) and then incubated in a CO 2 -free incubator at 37°C for 1 h. The cells were then physically dissociated from the culture dishes, stained with Alexa Flour conjugated 647 anti-rat CD11b antibody (Biolegend) and analyzed on a BD AccuriTM C6 flow cytometer (BD biosciences).

Techniques: Plasmid Preparation, Recombinant, Isolation, cDNA Synthesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Biology

Article Title: Structural Sampling of Glycan Interaction Profiles Reveals Mucosal Receptors for Fimbrial Adhesins of Enterotoxigenic Escherichia coli

doi: 10.3390/biology2030894

Figure Lengend Snippet: ( a ) Natural glycan array screening for F17G(4Lys) variants a (upper) and b (lower); ( b ) Natural glycan array concentration-dependent screening for F17bG with His-tag. Mouse anti-His antibodies, instead of polyclonal rabbit anti-F17G, were here used as primary antibodies, in order to reduce the background level observed in ( a ) for F17bG.

Article Snippet: Detection of bound lectin domains was achieved with a typical sandwich assay, in which the primary antibody was always a rabbit polyclonal IgG (10 μg/mL, in PBS with 0.01% Tween20 and 1% BSA), incubated for 45 min at room temperature, followed by incubation with mouse anti-rabbit AlexaFluo555 labeled antibodies (at 1:1,000 dilution, Molecular Probes).

Techniques: Glycoproteomics, Concentration Assay